COSTS ARE INDICATIVE (2012). PLEASE INQUIRE FOR EXACTS PRICES
Zebrafish line maintenance (transient line from DNA injection, mutant, stable transgene)
Service unit: 10 fish/tank, 365 days
Full cost per unit: 500
Cost justification:
Continue water and food supply 150
Instruments depreciation 100
Personnel (feeding, cleaning, patrolling) 200
Overheads 10% 50
Zebrafish line acquisition from foreign stock centre or laboratory
Service unit: Order of one line of zebrafish
Full cost per unit: 445
Cost justification:
Personnel 100
Shipmement 300
Overheads 10% 45
Training on genotyping
Full cost per unit: 450
Cost justification:
Material and consumables supplies 50 (oligos ordering excluded)
Instrumentation depreciation 100
Personnel 250
Overheads 10% 50
Training on zebrafish biology, maintenance and breeding
Full cost per unit : 250
Cost justification:
Personnel salaries (10 hrs) 225
overheads 10% 25
Training on whole mount in situ hybridization
Full cost per unit: 1600
Cost justification
Material and consumables supplies 300
Instrumentation depreciation 300
Personnel 850
Overheads 10% 150
Training on morpholino technology: preparation of eggs, microinjection, incubation, in vivo observation and fixation of embryos at specific stages. Standard oligos: specific oligos must be ordered (under supervision) by the customer.
Full cost per unit: 800
Cost justification
Material and consumables supplies (morpholino ordering excluded) 200
Instrumentation depreciation 250
Personnel 300
Overheads 10% 50
Training on germ line transformation by Tol2 mediated transgenesis: preparation of eggs, microinjection, incubation, in vivo observation.
Full cost per unit: 800
Cost justification
Material and consumables supplies (plasmid cloning excluded) 200
Instrumentation depreciation 250
Personnel 300
Overheads 10% 50
Training on mRNA injection: preparation of eggs, microinjection, incubation, in vivo observation, fixation at specific stages.
Full cost per unit: 1000
Cost justification
Material and consumables supplies (probe included) 400
Instrumentation depreciation 200
Personnel 300
Overheads 10% 100
Eggs, larvae, embryos for analysis (wild type), > 50 alive or fixed at specific stage released in Padova
Full cost per clutch 100
Cost justification
Material and consumables supplies 15
Instrumentation depreciation 15
Personnel 60
Overheads 10% 10
Eggs, larvae, embryos for analysis (specific strain present in Padova)> 50 alive or fixed at specific stage. Released in Padova
Full cost per clutch: 200
Cost justification
Material and consumables supplies 50 (genotyping required)
Instrumentation depreciation 30
Personnel 100
