Publications

2011

De Franceschi G, Frare E, Pivato M, Relini A, Penco A, Greggio E, Bubacco L, Fontana A, de Laureto PP
"Structural and morphological characterization of aggregates species of alpha-synuclein induced by docosahexaenoic acid."
Journal of Biological Chemistry, 2011, accepted

Aioanei D, Lv S, Tessari I, Rampioni A, Bubacco L, Li H, Samorì B, Brucale M.
"Single-molecule-level evidence for the osmophobic effect."
Angewandte Chemie International Edition, 2011, 50, 4394-4397.

Greggio E, Bisaglia M, Civiero L, Bubacco L.
"Leucine-rich repeat kinase 2 and alpha-synuclein: intersecting pathways in the pathogenesis of parkinson’s disease."
Molecular neurodegeneration, 2011, 6:6

Benetti F, Micetic I, Carsughi F, Spinozzi F, Bubacco L, Beltramini M.
"Insights into the oligomerization process of the C-terminal domain of human plasma membrane Ca
2+-ATPase."
 Archives of Biochemistry and Biophysics, 2011, 506, 194-200.

Marino SM, Fogal S, Bisaglia M, Moro S, Scartabelli G, De Gioia L, Spada A,  Monzani E, Casella L, Mammi S, Bubacco L.
"Investigation of Streptomyces antibioticus tyrosinase reactivity toward chlorophenols."
Archives of Biochemistry and Biophysics, 2011, 505, 67-74.

2010

Bortolus M, Bisaglia M, Zoleo A, Fittipaldi M, Benfatto M, Bubacco L, Maniero AL.
"Structural characterization of a high affinity mononuclear site in the copper(II)-alpha-Synuclein complex."
Journal of American Chemistry Society 2010, 132, 18057-18066.

Bisaglia M, Soriano ME, Arduini I, Mammi S and Bubacco L.
"Molecular characterization of dopamine-derived quinones reactivity toward NADH and glutathione: implications for mitochondrial dysfunction in Parkinson disease."
Biochimica et Biophysica Acta, 2010, 1802, 699-706.

Hirota S, Tanaka N, Micetic I, Di Muro P, Nagao S, Kitagishi H, Kano K, Magliozzo RS, Peisach J, Beltramini M, Bubacco L.
"Structural basis of the lactate-dependent allosteric regulation of oxygen binding in arthropod hemocyanin."
Journal of Biological Chemistry, 2010, 285, 19338-19345.

Bisaglia M, Greggio E, Maric D, Miller DW, Cookson MR and Bubacco L.
"Alpha-synuclein overexpression increases dopamine toxicity in BE2-M17 cells."
BMC Neuroscience, 2010, 11:41.

 Bisaglia M, Tosatto L, Munari F, Tessari I, de Laureto PP, Mammi S and Bubacco L.
"Dopamine quinones interact with alpha-synuclein to form unstructured adducts."
Biochememical and Biophysical Research Communication, (2010), 394, 424-428.

2009

De Franceschi G, Frare E, Bubacco L, Mammi S, Fontana A and de Laureto PP.
"Molecular Insights into the Interaction between alpha-Synuclein and Docosahexaenoic Acid"
Journal of Molecular Biology, 2009, 394, 94-107.

Marco Bisaglia, Isabella Tessari, Stefano Mammi and Luigi Bubacco
"Interaction Between alpha-Synuclein and Metal Ions, Still Looking for a Role in the Pathogenesis of Parkinson's Disease"
Neuromolecular Medicine, 2009, 11, 239-251.

Alessia Spada, Sara Palavicini, Enrico Monzani, Luigi Bubacco and Luigi Casella,
"Trapping tyrosinase key active intermediate under turnover"
Dalton Transactions, 2009, 33, 6468-6471.

Strianese M, Zauner G, Tepper AW, Bubacco L, Breukink E, Aartsma TJ, Canters GW and Tabares LC
"A protein-based oxygen biosensor for high-throughput monitoring of cell growth and cell viability"
Analytical Biochemistry 2009, 385, 242-248.

Brucale M, Sandal M, Di Maio S, Rampioni A, Tessari I, Tosatto L, Bisaglia M, Bubacco L and Samorì B
"Pathogenic mutations shift the equilibria of
α-Synuclein single molecule towards structured conformers"
ChemBioChem 2009, 10, 176-183.

Bisaglia M, Mammi S and Bubacco L
"Structural insights on the physiological functions and pathological effects of
α-synuclein"
FASEB Journal 2009, 23, 329-340.

2008

Hirota S. Kawahara T, Beltramini M,  Di Muro P, Magliozzo RS, Peisach J, Powers LS,  Tanaka N, Nagao S and Bubacco L "Molecular Basis of the Bohr Effect in Arthropod Hemocyanin"
Journal of Biological Chemistry 2008, 283, 31941-31948.

Vanin S, Bubacco L and Beltramini M
"Seasonal variation of the Trehalose and glycerol concentration in winter-snow active insects"
CryoLetters  2008, 29, 485-491.

Zauner G, Strianese M, Bubacco L, Aartsma TJ, Tepper AWJW and Canters GW
"Type-3 copper proteins as biocompatible and reusable oxygen sensors"
 Inorganica Chemica Acta 2008, 361, 1116-1121.

Bisaglia M, Mammi S and Bubacco L.
"Structures and interactions of
α-synuclein. Physiological and pathological relevance"
in Biophysical Inquiry into Protein Aggregation and Amyloid Diseases, TRIVANDRUM: Transworld Research Network (2008) 155-180 (ISBN 978-81-7895-354-0).

Bortolus M, Tombolato F, Tessari I, Bisaglia M, Mammi S, Bubacco L, Ferrarini A and Maniero A.
"Broken helix in vesicle and micelle-bound
α-Synuclein: insights from SDSL-EPR experiments and MD simulations"
Journal of American Chemistry Society 2008, 130, 6690-6691.

Tessari I, Bisaglia M, Valle F, Samorì B, Bergantino E, Mammi S and Bubacco L.
"The reaction of
α-synuclein with tyrosinase: Possible implications for Parkinson disease"
Journal of Biological Chemistry 2008, 283, 16808-16817.

Campello S, Beltramini M, Giordano G, Di Muro P, Marino SM, Bubacco L.
"Role of the tertiary structure in the diphenol oxidase activity of Octopus vulgaris hemocyanin"
Arch Biochem Biophys 2008, 471, 159-167.

Sandal M, Valle F, Tessari I, Mammi S, Bergantino E, Musiani F, Bubacco L and Samorì B.
"Probing the conformational diversity of the monomeric
α-synuclein at the single molecule level"
PLoS Biology 2008, 6, e6.

2007

Bubacco L, Spinazze R, della Longa S, Benfatto M.
"X-ray absorption analysis of the active site of Streptomyces antibioticus Tyrosinase upon binding of transition state analogue inhibitors"
Arch Biochem Biophys 2007, 465, 320-327.

Zauner, G., Lonardi, E., Bubacco, L., Aartsma, T.J., Canters G.W., Tepper A.
"Oxygen Sensing using Type-3 Copper Proteins and Tryptophan-to-Dye Fluorescence Energy Transfer"
Chemistry 2007, 13, 7085-7090.

Marino, S., Hayakawa, K., Hatada, K., Benfatto, M., Rizzello, A., Maffia, M., Bubacco L.
"Structural features that govern enzymatic activity in Carbonic Anhydrase from a  low temperature adapted fish Chionodraco hamatus"
Biophysical Journal 2007, 93, 2781-2790.

Bisaglia, M., Mammi S., Bubacco L.
"Kinetic analysis of the rearrangement of aminochrome by NMR spectroscopy"
 J Biol Chem, 2007, 282, 15597-15605.

2006

Tepper, A., Bubacco, L., Canters, G.W.
"Paramagnetic properties of the halide bound derivatives of oxidised tyrosinase investigated by 1H NMR spectroscopy"
Chemistry, 2006, 12, 7668-7675.

Bisaglia, M., Trolio, A., Bellanda, M., Bergantino, E., Bubacco, L., Mammi S.
"Structure and Topology of the non A
myloid-β Component Fragment of Human α-Synuclein Bound to Micelles:  Implications for the Aggregation Process"
Protein Science 2006, 15,1408-1416.

Vanin, S., Negrisolo, E., Bailly, I.,  Bubacco, L., Beltramini, M., Salvato, B.
"Molecular evolution and phylogeny of Sipunculean Hemerythrins"
Journal of Molecular Evolution 2006, 62, 32-41.

Granata, A., Monzani,  E., Bubacco,  L., Casella L.
"Mechanistic insight into the activity of tyrosinase from variable temperature studies in aqueous-organic solvent"
Chemistry, 2006, 12, 2504-2514.

2005

Sarangi, R., Benfatto, M., Hayakawa, K., Bubacco, L., Solomon, E.I., Hodgson, K.O., Hedman, B.
"MXAN Analysis of the XANES Energy Region of a Mononuclear Copper Complex: Applications to Bioinorganic Systems"
Inorganic Chemistry 2005, 44, 9652-9659.

Beltramini, M., Colangelo, N., Giomi, F., Bubacco, L., Di Muro, P., Hellmann, N., Jaenicke, E., Decker, H.
"Quaternary structure and functional properties of Penaeus monodon hemocyanin"
FEBS Journal
2005, 272, 2060-2075.

 Greggio, E.,  Bergantino, E., Carter, D., Ahma, R., Costin, G., Hearing, V., Clarimon, J., Singleton, A., Eerola, J., Hellström, O., Tienari, P., Miller, D., Beilina, A., Bubacco, L., Cookson, M.
"
Tyrosinase exacerbates dopamine and α-synuclein toxicity but is not genetically associated with Parkinson’s disease"
Journal of Neurochemistry 2005, 93, 246-256.

Tepper, A., Bubacco L., Canters G.W.
"Interaction between the Type-3 Copper Protein Tyrosinase and the Substrate Analogue p-Nitrophenol Studied by NMR"
 Journal of the American Chemical Society 2005, 127, 567-575

Bisaglia, M., Tessari, I., Pinato, L., Bellanda, M., Giraudo, S., Fasano, M., Bergantino, E., Bubacco, L., Mammi, S.
"A Topological Model of the Interaction between
α-Synuclein and Sodium Dodecyl Sulfate Micelles"
Biochemistry 2005, 44, 329-339.

Bisaglia, M., Trolio, A., Tessari, I., Bubacco, L., Mammi, S., Bergantino E.,
"Cloning, expression, purification and spectroscopic analy
sis of α-fragment of human α-synuclein"
Protein Expr Purif 2005, 39, 90-96.

2004

Casella L., Granata E., Monzani E., Pievo R., Bubacco L.
"New aspects of the reactivity of tyrosinase"
MICRON 2004, 35, 141-142.

Bubacco L., Van Gastel M., Benfatto M., Tepper, A., Canters G.W.
"What are the structural features of the active site that define binuclear copper proteins function?"   
MICRON 2004, 35, 143-145.

Tepper, A., Bubacco, L., Canters, G.W.,
"Stopped-flow fluorescence studies of  inhibitor binding to tyrosinase from Streptomyces antibioticus
J Biol Chem 2004, 279, 13425-13434.

2003

Bubacco L., Van Gastel M., Groenen E.J., Vijgenboom E., Canters G.W.
"Spectroscopic characterization of the electronic changes in the active site of Streptomyces antibioticus tyrosinase upon binding of transition state analogue inhibitors"
J Biol Chem 2003, 278, 7381-7389.

2002

Battaini, G., Monzani, E., Casella, L., Lonardi, E., Tepper, A., Canters, G.W.  Bubacco, L.
"Tyrosinase catalyzed oxidation of fluorophenols"
J Biol Chem 2002, 277, 44606-44612.

Tepper, A.W.J.W., Bubacco, L., Canters, G.W.,
"Structural basis and mechanism of the inhibition of the type-3 copper protein tyrosinase from Streptomyces antibioticus by halide ions"
J Biol Chem 2002, 277, 30436-30444.

Borghi, E., Solari P.L., Beltramini, M., Bubacco, L., Di Muro, P., Salvato, B.,
"Oxidised derivatives of Octopus vulgaris and Carcinus aestuarii hemocyanins at pH 7.5 and related models by X-ray absorption spectroscopy"
Biophysical Journal 2002, 82, 3245-3268.

Sabatucci, A., Ascone, I., Bubacco, L., Beltramini, M., Di Muro, P., Salvato, B.
"Comparison of the X-ray absorption properties of the binuclear active site of molluscan and arthropodan hemocyanins"
JBIC 2002, 7, 120-128.

2001

Bubacco, L., Mardegan, A., Giordano, G., van Gastel , M., Tepper, A., Canters, G.W.,
"Structure and reactivity of the binuclear copper center in Streptomyces antibioticus Tyrosinase"
J.Inorg.Biochem.
2001, 86, 28-28.

2000

Molon, A., Di Muro P., Bubacco L., Vasilyev V., Salvato B., Beltramini M., Conze W., Hellmann N., Decker H.
"Molecular heterogeneity of the Hemocyanin isolated from the king crab Paralithodes camtschaticae"
European Journal of Biochemistry
2000, 267, 7046-7057.

Bubacco L.
"Electron Spin Echo Envelope Modulation Spectroscopy (ESEEM) in the study of Biological systems"
in Spectroscopic Techniques in Biophysics G.M. Giacometti and G. Giacometti Eds., pp1-26,  IOS Press 2000.

van Gastel M., Bubacco, L., Groenen E., Vijgenboom, E., Canters, G.W.
"EPR study of the dinuclear active site of Tyrosinase from Streptomyces antibioticus"
FEBS Letter 2000, 474, 228-232.

Ascone I., Sabatucci A., Bubacco L., Di Muro, P., Salvato, B.
"Saccharose solid matrix embedded proteins : a new methods for sample preparation for X-ray absorption spectroscopy"
European Journal of Biophysics 2000, 29, 391-397.

Holm, J.K., Hemmingsen, L., Bubacco, L., Salvato, B., Bauer, R.
"Interaction and coordination geometries for Ag(I) in the two metal site of hemocyanin"
European Journal of Biochemistry 2000, 267, 1-8.

Bubacco, L., Vijgenboom, E., Gobin, C., Tepper, A.., Salgado, J., Canters, G.W.
"Paramagnetic NMR investigation of the binding of transition state analogue inhibitors to the dinuclear copper site of Streptomyces antibioticus Tyrosinase"
Journal of Molecular Catalysis B: Enzymatic 2000, 8, 27-35.

1990-1999

Bubacco, L., Salgado, J., Vijgenboom, E., Canters, G.W.
"Characterisation by proton NMR spectroscopy of the binuclear site of Streptomyces antibioticus Tyrosinase"
FEBS Letter 1999, 442, 215-220.

Zlateva, T., Santagostini , L., Bubacco, L., Casella, L., Salvato, B., Beltramini, M.
"Isolation of a met-derivative intermediate in the hydrogen peroxide dismutase activity of deoxygenated Octopus vulgaris hemocyanin"
 J. Inorg. Biochem 1998, 72, 211-215.

Salgado, J., Warmerdam, G.,  Bubacco, L., Canters, G.W.
"Understanding the electronic properties of the CuA site from cytochrome c oxidase through paramagnetic 1H NMR"
Biochemistry 1998, 37, 7378-7389.

Alzuet, G., Bubacco, L., Casella, L., Rocco, G.P., Salvato, B., Beltramini, M.
"The binding of azide to copper and cobalt-containing forms of hemocyanin from the Mediterranean crab Carcinus aestuarii"
European Journal of Biochemistry 1997, 247, 688-694.

Huber, M., Bubacco, L., Beltramini, M., Salvato, B.  Peisach, j.,  Haase, W.
"Cobalt (II) substituted derivative of Carcinus maenas hemocyanin: Magnetic characterization, magnetooptic, and Kinetics studies regarding the geometry of the  active site"
Inorganic Chemistry 1996, 35, 7882-7492.

Bubacco, L., Magliozzo, R.S.,  Beltramini, M., Peisach, J., Salvato, B.
"Nitrate reductase activity of the deoxy Carcinus maenas hemocyanin: the formation of the half-met derivative "
Inorganic Chemistry
1996, 35, 1393-1394.

Beltramini, M., Bubacco, L., Casella, L., Alzuet, G., Gullotti, M., Salvato, B.
"The oxidation of hemocyanin: kinetics, reaction mechanism and characterization of the met-hemocyanin  product"
European Journal of Biochemistry 1995, 232, 98-105.

Wirt, M.D., Bubacco, L., Peisach, J.
"Investigation of the solid and solution structures of N-substituted Cu(II) salicyldimines by x-ray  absorption spectroscopy" 
Inorganic Chemistry 1995, 34, 2377-2381.

Magliozzo, R.S., Bubacco, L., Mc Crachen,  Jang, F., Beltramini, M., Salvato, B.,  Peisach,J.
"Cu(II) coordination in Arthropods and Mollusc green half-met hemocyanin analysed  by  electron  spin echo envelope modulation spectroscopy"
Biochemistry 1995, 34, 1511-1531.

Bubacco, L., Magliozzo, R. S, Wirt, M.D.,  Beltramini, M., Salvato, B., Peisach,J.
"Structural characterization of mononuclear Cu(II) in the active site of Carcinus maenas hemocyanin"
Biochemistry
1995, 34, 1524-1533.

Della Longa, S., Bianconi, A., Palladino, L., Cangiu-Castellano, A., Simonelli, B., Barteri, M.,  Borghi, E., Beltramini, M., Rocco, G.P., Salvato, B., Bubacco, L., Magliozzo, R.S.,  Peisach, J.
"XANES study of the metal coordination geometry in Co(II)-substituted  Carcinus maenas hemocyanin"
Biophysical Journal 1993, 65, 2680-269.

Jang, F., Conry, R.R., Bubacco, L., Tyeklar, Z., Jacobson, R.R., Karlin, K.D., Peisach, J.
"Crystal structure end electron spin echo modulation (ESEEM) study of [Cu(II)(TEPA)NO2]PF6 (TEPA)=Tris[2-(2-pyridyl)ethyl] amine): A model for the  purported structure of the nitrite derivative of hemocyanin"
Journal of the American  Chemical Society 1993, 115, 2093-2102.

Bubacco, L., Rocco. G.P., Salvato, B., Beltramini, M.
"The binding of Cd(II) to the hemocyanin of the Mediterranean Crab Carcinus maenas
Archives of Biochemistry and Biophysics 1993, 302, 78-84.

 Vitrano, E., Cupane, A., Leone, M., Militello, V., Cordone, L., Salvato, B., Beltramini, M., Bubacco, L., Rocco, G.P.
"Low temperature spectroscopy of cobalt-substituted hemocyanin from Carcinus maenas"
European Journal of Biophysics 1993, 22, 157-167.

Bubacco, L., Beltramini, M., Salvato, B., Magliozzo, R.S., Peisach, J.
"Preparation and spectroscopic characterization of a coupled binuclear center in Co(II)- substituted hemocyanin"
Biochemistry 1992, 31, 9294-9303.

Beltramini, M., Bubacco, L., Salvato, B., Casella, L., Gullotti, M., Garofani, S.
"The aromatic circular dichroism as a probe for conformational changes in the active site  environment of hemocyanin"
Biochimica and Biophysica Acta 1992, 1120, 24- 32.

Ricchelli, F., Beltramini, M., Bubacco, L., Salvato, B., Filippi, B.
"Circular dichroism and fluorescence studies to probe the conformational properties of Rhus vernicifera laccase"
Inorganica Chimica Acta
1992, 193, 237-243.

Bubacco, L., Beltramini, M., Salvato, B., Magliozzo, R.S.
"Cobalt-substituted derivatives of Carcinus hemocyanin"
Biology of Metal 1990, 3, 90-92.

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