Marco Bisaglia, PhD

 

Department of Biology
University of Padova
Via Ugo Bassi, 58/B
35121 Padova-Italy

Tel.: +39/049/8276329
Fax: +39/049/8276300
E-mail: marco.bisaglia@unipd.it

_______________________________________________________________________________________________

 

RESEARCH INTERESTS

Oxidative stress in Parkinson disease.
Structure-function relationships of human
a-synuclein.

 

METHODS

NMR spectroscopy.
Molecular biology.
Biochemical and biophysical techniques.

 

EDUCATION

Ph.D. (cum laude) in Structural Biology, Polytechnic School, Paris, France (2002)
Supervisor: Dr. Franįois Bontems
Structural and Functional Characterization of two proteins involved in mRNA metabolism.

Master Degree in Chemistry, University of Padova, Italy, (1999)
Supervisor: Prof. Stefano Mammi
Point-mutated hybrids of 1-34 parathyroid hormone-related protein (PTHrP): conformational studies in a membrane-mimetic environment.

Erasmus Internship in Metallorganic Chemistry, University of Versailles, France, (1997)
Supervisor: Dr. Dominique Vichard
Synthesis of
p-complexed nitrobenzene derived compounds. Applications in the case of aromatic nucleophilic substitutions.

 

FELLOWSHIPS AND AWARDS:

European Molecular Biology Organization (EMBO), Three months short term fellowship, 2007.
GIDRM conference, 1st place (2006) and 3rd place (2003, 2004) awards in poster competition.
University of Padova, Research postdoctoral fellowship, 2003-2008.
French Foundation for the Medical Research (FRM), 3rd year Doctoral fellowship, 2002.
French National Center of Scientific Research (CNRS), Annual fellowship, 2001.
Polytechnic School, Seven-months visitor fellowship, 2000.
University of Padova, Six-months fellowship, 1999.
University of Padova, Three months Erasmus fellowship, 1997.

 

PUBLICATIONS:

Greggio E, Bisaglia M, Civiero L, Bubacco L.
Leucine-rich repeat kinase 2 and alpha-synuclein: intersecting pathways in the pathogenesis of parkinson’s disease.
Molecular neurodegeneration (2011), 6:6

Marino SM, Fogal S, Bisaglia M, Moro S, Scartabelli G, De Gioia L, Spada A, Monzani E, Casella L, Mammi S, Bubacco L.
Investigation of Streptomyces antibioticus tyrosinase reactivity toward chlorophenols.
Archives of Biochemistry and Biophysics (2011).

Bortolus M, Bisaglia M, Zoleo A, Fittipaldi M, Benfatto M, Bubacco L, Maniero AL.
Structural characterization of a high affinity mononuclear site in the copper(II)-alpha-Synuclein complex.
J
ournal of American Chemistry Society (2010), 132, 18057-18066.

Bisaglia M, Soriano ME, Arduini I, Mammi S, Bubacco L.
Molecular characterization of dopamine-derived quinones reactivity toward NADH and glutathione: implications for mitochondrial dysfunction in Parkinson disease.
Biochimica et Biophysica Acta (2010), 1802, 699-706.

Bisaglia M, Greggio E, Maric D, Miller DW, Cookson MR and Bubacco L.
Alpha-synuclein overexpression increases dopamine toxicity in BE2-M17 cells.
BMC Neuroscience (2010), 11:41.

 Bisaglia M, Tosatto L, Munari F, Tessari I, de Laureto PP, Mammi S and Bubacco L.
Dopamine quinones interact with alpha-synuclein to form unstructured adducts.
Biochememical and Biophysical Research Communication (2010), 394, 424-428.

Bisaglia M, Tessari I, Mammi  S and Bubacco L
Interaction Between alpha-Synuclein and Metal Ions, Still Looking for a Role in the Pathogenesis of Parkinson's Disease
Neuromolecular Medicine (2009), 11, 239-251.

Salah P, Bisaglia M, Aliprandi P, Uzan M, Sizun C and Bontems F
Probing the relationship between Gram-negative and Gram-positive S1 proteins by sequence analysis
Nucleic Acid Research (2009), 37, 5578-5588.

Brucale M, Sandal M, Di Maio S, Rampioni A, Tessari I, Tosatto L, Bisaglia M, Bubacco L and Samorė B
Pathogenic mutations shift the equilibria of
α-Synuclein single molecule towards structured conformers
ChemBioChem (2009), 10, 176-183.

Bisaglia M, Mammi S and Bubacco L
Structural insights on physiological functions and pathological effects of alpha-Synuclein
FASEB Journal (2009), 23, 329-340.

Bortolus M, Tombolato F, Tessari I, Bisaglia M, Mammi S, Bubacco L, Ferrarini A and Maniero A
Broken helix in vesicle and micelle-bound alpha-Synuclein: insights from SDSL-EPR experiments and MD simulations
Journal of American Chemistry Society (2008), 130, 6690-6691.

Tessari I, Bisaglia M, Valle F, Samorė B, Bergantino E, Mammi S and Bubacco L
The reaction of alpha-synuclein with tyrosinase: Possible implications for Parkinson disease
Journal of Biological Chemistry (2008), 283, 16808-16817.

Bisaglia M, Mammi S and Bubacco L
Structures and interactions of alpha-synuclein. Physiological and pathological relevance
in "Biophysical Inquiry into Protein Aggregation and Amyloid Diseases" TRIVANDRUM: Transworld Research Network (2008), 155-180, ISBN 978-81-7895-354-0.

Bisaglia M, Mammi S and Bubacco L
Kinetic and structural analysis of the early oxidation products of dopamine. Analysis of the interactions with alpha-synuclein
Journal of Biological Chemistry (2007), 282, 15597-15605.

Durand S, Richard G, Bisaglia M, Laalami S, Bontems F and Uzan M
Activation of RegB endoribonuclease by S1 ribosomal protein requires an 11 nt conserved sequence
Nucleic Acid Research (2006), 34, 6549-6560.

Bisaglia M, Trolio A, Bellanda M, Bergantino E, Bubacco L and Mammi S
Structure and topology of the non amyloid-beta component fragment of human alpha-synuclein bound to micelles: implications for the aggregation process
Protein Science (2006), 15, 1408-1416.

Bisaglia M, Schievano E, Caporale A, Peggion E and Mammi S
The 11-mer repeats of human alpha-synuclein in vesicle interactions and lipid composition discrimination: a cooperative role
Biopolymers (2006), 84, 310-316.

Bisaglia M, Tessari I, Pinato L, Bellanda M, Giraudo S, Fasano M, Bergantino E, Bubacco L and Mammi S
A Topological Model of the Interaction between alpha-Synuclein and SDS Micelles
Biochemistry (2005), 44, 329-339.

Bisaglia M, Trolio A, Tessari I, Bubacco L, Mammi S and Bergantino E
Cloning, expression, purification and spectroscopic analysis of the fragment 57-102 of human alpha-
synuclein
Protein Expression and Purification (2005), 39, 90-96.

Bisaglia M, Laalami S, Uzan M and Bontems F
Activation of the RegB Endoribonuclease by the S1 Ribosomal Protein Is Due to Cooperation between the S1 Four C-terminal Modules in a Substrate-dependant Manner
Journal of Biological Chemistry (2003), 278, 15261-71.

 

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